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Image Search Results
Journal: F1000Research
Article Title: Murine Tim-1 is excluded from the immunological synapse
doi: 10.12688/f1000research.1-10.v2
Figure Lengend Snippet: ( A ) Jurkat T cells transfected with empty vector (EV), Flag-Tim-1, or Flag-Tim-1 QGQ were stimulated with anti-TCR and anti-CD28 antibodies for the indicated times. Lysates were analyzed by SDS-PAGE and western blotting for pY (4G10), pSrc (Y416; analogous to Y394 in Lck), and β-actin. ( B ) Jurkat T cells were stimulated with anti-CD3 mAb for the indicated times. CD3 expression was measured with flow cytometry and mean fluorescence intensity (MFI) was determined in FloJo. Dynasore (DS, 80 µM) was used to prevent clathrin-mediated endocytosis after TCR/CD3 crosslinking.
Article Snippet: The following antibodies were used:
Techniques: Transfection, Plasmid Preparation, SDS Page, Western Blot, Expressing, Flow Cytometry, Fluorescence