phospho-src (tyrosine 416) antibody Search Results


97
Cell Signaling Technology Inc rabbit a phospho src family
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Cell Signaling Technology Inc rabbit antiphosphotyrosine 416 src
Rabbit Antiphosphotyrosine 416 Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc psrc y416 antibody
( A ) Jurkat T cells transfected with empty vector (EV), Flag-Tim-1, or Flag-Tim-1 QGQ were stimulated with anti-TCR and anti-CD28 antibodies for the indicated times. Lysates were analyzed by SDS-PAGE and western blotting for pY (4G10), <t>pSrc</t> <t>(Y416;</t> analogous to Y394 in Lck), and β-actin. ( B ) Jurkat T cells were stimulated with anti-CD3 mAb for the indicated times. CD3 expression was measured with flow cytometry and mean fluorescence intensity (MFI) was determined in FloJo. Dynasore (DS, 80 µM) was used to prevent clathrin-mediated endocytosis after TCR/CD3 crosslinking.
Psrc Y416 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc rabbit anti phosphotyrosine src
( A ) Jurkat T cells transfected with empty vector (EV), Flag-Tim-1, or Flag-Tim-1 QGQ were stimulated with anti-TCR and anti-CD28 antibodies for the indicated times. Lysates were analyzed by SDS-PAGE and western blotting for pY (4G10), <t>pSrc</t> <t>(Y416;</t> analogous to Y394 in Lck), and β-actin. ( B ) Jurkat T cells were stimulated with anti-CD3 mAb for the indicated times. CD3 expression was measured with flow cytometry and mean fluorescence intensity (MFI) was determined in FloJo. Dynasore (DS, 80 µM) was used to prevent clathrin-mediated endocytosis after TCR/CD3 crosslinking.
Rabbit Anti Phosphotyrosine Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti phosphotyrosine src - by Bioz Stars, 2026-03
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90
Cell Signaling Technology Inc antibodies to phospho-specific jak2 (tyr-1007/1008)
( A ) Jurkat T cells transfected with empty vector (EV), Flag-Tim-1, or Flag-Tim-1 QGQ were stimulated with anti-TCR and anti-CD28 antibodies for the indicated times. Lysates were analyzed by SDS-PAGE and western blotting for pY (4G10), <t>pSrc</t> <t>(Y416;</t> analogous to Y394 in Lck), and β-actin. ( B ) Jurkat T cells were stimulated with anti-CD3 mAb for the indicated times. CD3 expression was measured with flow cytometry and mean fluorescence intensity (MFI) was determined in FloJo. Dynasore (DS, 80 µM) was used to prevent clathrin-mediated endocytosis after TCR/CD3 crosslinking.
Antibodies To Phospho Specific Jak2 (Tyr 1007/1008), supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Cell Signaling Technology Inc rabbit polyclonal anti phospho src
( A ) Jurkat T cells transfected with empty vector (EV), Flag-Tim-1, or Flag-Tim-1 QGQ were stimulated with anti-TCR and anti-CD28 antibodies for the indicated times. Lysates were analyzed by SDS-PAGE and western blotting for pY (4G10), <t>pSrc</t> <t>(Y416;</t> analogous to Y394 in Lck), and β-actin. ( B ) Jurkat T cells were stimulated with anti-CD3 mAb for the indicated times. CD3 expression was measured with flow cytometry and mean fluorescence intensity (MFI) was determined in FloJo. Dynasore (DS, 80 µM) was used to prevent clathrin-mediated endocytosis after TCR/CD3 crosslinking.
Rabbit Polyclonal Anti Phospho Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc anti-sfk antibody non-phospho-sfk (tyr 416) (7g9)
( A ) Jurkat T cells transfected with empty vector (EV), Flag-Tim-1, or Flag-Tim-1 QGQ were stimulated with anti-TCR and anti-CD28 antibodies for the indicated times. Lysates were analyzed by SDS-PAGE and western blotting for pY (4G10), <t>pSrc</t> <t>(Y416;</t> analogous to Y394 in Lck), and β-actin. ( B ) Jurkat T cells were stimulated with anti-CD3 mAb for the indicated times. CD3 expression was measured with flow cytometry and mean fluorescence intensity (MFI) was determined in FloJo. Dynasore (DS, 80 µM) was used to prevent clathrin-mediated endocytosis after TCR/CD3 crosslinking.
Anti Sfk Antibody Non Phospho Sfk (Tyr 416) (7g9), supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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92
Cell Signaling Technology Inc anti non phospho src
( A ) Jurkat T cells transfected with empty vector (EV), Flag-Tim-1, or Flag-Tim-1 QGQ were stimulated with anti-TCR and anti-CD28 antibodies for the indicated times. Lysates were analyzed by SDS-PAGE and western blotting for pY (4G10), <t>pSrc</t> <t>(Y416;</t> analogous to Y394 in Lck), and β-actin. ( B ) Jurkat T cells were stimulated with anti-CD3 mAb for the indicated times. CD3 expression was measured with flow cytometry and mean fluorescence intensity (MFI) was determined in FloJo. Dynasore (DS, 80 µM) was used to prevent clathrin-mediated endocytosis after TCR/CD3 crosslinking.
Anti Non Phospho Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ABclonal Biotechnology anti-phospho-src (tyr-416
( A ) Jurkat T cells transfected with empty vector (EV), Flag-Tim-1, or Flag-Tim-1 QGQ were stimulated with anti-TCR and anti-CD28 antibodies for the indicated times. Lysates were analyzed by SDS-PAGE and western blotting for pY (4G10), <t>pSrc</t> <t>(Y416;</t> analogous to Y394 in Lck), and β-actin. ( B ) Jurkat T cells were stimulated with anti-CD3 mAb for the indicated times. CD3 expression was measured with flow cytometry and mean fluorescence intensity (MFI) was determined in FloJo. Dynasore (DS, 80 µM) was used to prevent clathrin-mediated endocytosis after TCR/CD3 crosslinking.
Anti Phospho Src (Tyr 416, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc phosphor src
( A ) Jurkat T cells transfected with empty vector (EV), Flag-Tim-1, or Flag-Tim-1 QGQ were stimulated with anti-TCR and anti-CD28 antibodies for the indicated times. Lysates were analyzed by SDS-PAGE and western blotting for pY (4G10), <t>pSrc</t> <t>(Y416;</t> analogous to Y394 in Lck), and β-actin. ( B ) Jurkat T cells were stimulated with anti-CD3 mAb for the indicated times. CD3 expression was measured with flow cytometry and mean fluorescence intensity (MFI) was determined in FloJo. Dynasore (DS, 80 µM) was used to prevent clathrin-mediated endocytosis after TCR/CD3 crosslinking.
Phosphor Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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95
Cell Signaling Technology Inc p src
( A ) Jurkat T cells transfected with empty vector (EV), Flag-Tim-1, or Flag-Tim-1 QGQ were stimulated with anti-TCR and anti-CD28 antibodies for the indicated times. Lysates were analyzed by SDS-PAGE and western blotting for pY (4G10), <t>pSrc</t> <t>(Y416;</t> analogous to Y394 in Lck), and β-actin. ( B ) Jurkat T cells were stimulated with anti-CD3 mAb for the indicated times. CD3 expression was measured with flow cytometry and mean fluorescence intensity (MFI) was determined in FloJo. Dynasore (DS, 80 µM) was used to prevent clathrin-mediated endocytosis after TCR/CD3 crosslinking.
P Src, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Millipore anti-phospho src (tyr 416, 05-677)
( A ) Jurkat T cells transfected with empty vector (EV), Flag-Tim-1, or Flag-Tim-1 QGQ were stimulated with anti-TCR and anti-CD28 antibodies for the indicated times. Lysates were analyzed by SDS-PAGE and western blotting for pY (4G10), <t>pSrc</t> <t>(Y416;</t> analogous to Y394 in Lck), and β-actin. ( B ) Jurkat T cells were stimulated with anti-CD3 mAb for the indicated times. CD3 expression was measured with flow cytometry and mean fluorescence intensity (MFI) was determined in FloJo. Dynasore (DS, 80 µM) was used to prevent clathrin-mediated endocytosis after TCR/CD3 crosslinking.
Anti Phospho Src (Tyr 416, 05 677), supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) Jurkat T cells transfected with empty vector (EV), Flag-Tim-1, or Flag-Tim-1 QGQ were stimulated with anti-TCR and anti-CD28 antibodies for the indicated times. Lysates were analyzed by SDS-PAGE and western blotting for pY (4G10), pSrc (Y416; analogous to Y394 in Lck), and β-actin. ( B ) Jurkat T cells were stimulated with anti-CD3 mAb for the indicated times. CD3 expression was measured with flow cytometry and mean fluorescence intensity (MFI) was determined in FloJo. Dynasore (DS, 80 µM) was used to prevent clathrin-mediated endocytosis after TCR/CD3 crosslinking.

Journal: F1000Research

Article Title: Murine Tim-1 is excluded from the immunological synapse

doi: 10.12688/f1000research.1-10.v2

Figure Lengend Snippet: ( A ) Jurkat T cells transfected with empty vector (EV), Flag-Tim-1, or Flag-Tim-1 QGQ were stimulated with anti-TCR and anti-CD28 antibodies for the indicated times. Lysates were analyzed by SDS-PAGE and western blotting for pY (4G10), pSrc (Y416; analogous to Y394 in Lck), and β-actin. ( B ) Jurkat T cells were stimulated with anti-CD3 mAb for the indicated times. CD3 expression was measured with flow cytometry and mean fluorescence intensity (MFI) was determined in FloJo. Dynasore (DS, 80 µM) was used to prevent clathrin-mediated endocytosis after TCR/CD3 crosslinking.

Article Snippet: The following antibodies were used: pSrc Y416 and pZAP-70 Y319 (Cell Signaling), PKC-θ (C-18, Santa Cruz), CD43 (clone S7, BD Pharmingen), M2-Cy3 (Sigma Aldrich), EEA1 (BD Transduction), M2 anti-flag (Sigma Aldrich), anti-human CD3 (Becton Dickinson), mouse CD3 and CD28 (BD Pharmingen), human CD28 (Life Technologies), Tim-1 Fc (eBiosciences), anti-TCR antibody C305 (Harlan), anti-Tim-1 antibodies (3B3 and 5F12), anti-Tim-4 antibodies (3A1, 3H11 and 5G3).

Techniques: Transfection, Plasmid Preparation, SDS Page, Western Blot, Expressing, Flow Cytometry, Fluorescence